Vial sizes vary by vendor. Smaller bottles are usually 3 mL; larger bottles are often 10 mL. Match the label on the vial you actually bought, then use the calculator below if you need to adjust.
Overview
NAD+ is a coenzyme central to cellular energy metabolism, DNA repair, and sirtuin activation.
Preparation
Recon: 500 mg vial + 5 mL BAC (100 mg/mL).
Protocol
| Phase | Dose | Frequency | Duration |
|---|---|---|---|
| Loading | 100-250 mg (100 → 150 → 200 → 250 step-up) | Daily | 1-2 weeks |
| Maintenance | 100-250 mg | 2-3x/week | Ongoing |
Cycle
Loading followed by ongoing maintenance.
Research coverage
Related reporting from Compound Chronicle, which tracks the peer-reviewed literature on NAD+ Protocol.
- NAD+ and Frailty in Older Adults: New Human Evidence Sep 20, 2026
In 529 older adults, lower blood NAD+ was associated with frailty — human observational data from the Itabashi Longitudinal Study on Aging.
- NAD+ Research Is Widening — and So Are the Questions Aug 17, 2026
A batch of new NAD+ papers spans redox biology, cancer metabolism, and sirtuin signaling. The breadth is a reminder that NAD+ is a fundamental biochemistry topic, not just a supplement.